Laboratoire Eau Environnement et Systèmes Urbains (Leesu)

Recent publications

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Monitoring microplastics in the Seine River in the Greater Paris area
Cleo Stratmann, Rachid Dris, Johnny Gasperi, Frans Buschman, Adriaan Markus, Sabrina Guerin, A. Dick Vethaak, Bruno Tassin
, 2024, 12, ⟨10.3389/feart.2024.1386547⟩
Litter in French urban areas — Part 2: transport dynamic and fluxes in stormwater
Lauriane Ledieu, Romain Tramoy, David Mabilais, Sophie Ricordel, Marie-Laure Mosini, Alexandra Mosset, Bernard Flahaut, Laetitia Pineau, Zoé Bridant, Eric Bouchet, Clémence Bruttin, Fabrice Rodriguez, Bruno Tassin, Johnny Gasperi
, 2024, ⟨10.1007/s11356-024-33774-0⟩
Fluorescence spectroscopy for tracking microbiological contamination in urban waterbodies
Natália Angelotti de Ponte Rodrigues, Rémi Carmigniani, Arthur Guillot-Le Goff, Françoise S Lucas, Claire Therial, Manel Naloufi, Aurélie Janne, Francesco Piccioni, Mohamed Saad, Philippe Dubois, Brigitte Vinçon-Leite
, 2024, 6, ⟨10.3389/frwa.2024.1358483⟩
Séparation à la source des excrétats : bases pour des règles professionnelles
Florent Brun, Fabien Esculier, Bernard de Gouvello
, 2024, GE1029v1, ⟨10.51257/a-v1-ge1029⟩
Small-Size Microplastics in Urban Stormwater Runoff are Efficiently Trapped in a Bioretention Cell
Kelsey Smyth, Shuyao Tan, Tim van Seters, Johnny Gasperi, Rachid Dris, Jennifer Drake, Elodie Passeport
, 2024, ⟨10.1021/acsestwater.4c00037⟩

Supervisory authorities

Member of

WaterOmics presentation

by Daniel Thevenot, Julien Le Roux - published on , updated on

The WaterOmics research project (2017-2021) is funded by Agence Nationale de la Recherche (ANR) (JCJC program).

Its title is « Tracking organic micropollutants in urban waters by high resolution mass spectrometry: omics approaches and fingerprint indices »

Project leader: Julien Le Roux

In the key topic Micropollutants
In the research theme Functioning, resilience and adaptation of urban water management

Numerous emerging micropollutants (e.g., personal care products, antibiotics, endocrine disruptors) are found in urban waters but their transformation and behavior in wastewater treatment plants and in the urban environment are not well characterized. Current workflows for the non-targeted analysis of organic micropollutants by high resolution mass spectrometry (HRMS) are complex and time-consuming. The aim of this 4-year project is to develop a comprehensive approach for the characterization of organic micropollutants in urban waters by HRMS, by (1) providing an innovative protocol for the extraction and analysis of both polar and non-polar compounds from urban waters; (2) developing data treatment methods and global indices derived from HRMS fingerprints; and (3) applying chemometrics/omics methods for data analyses of urban water samples in conjunction with toxicity analyses, with a focus on the characterization of polar molecules.

The achievement of these objectives will provide advances in workflows for the detection of micropollutants using non-targeted analyses. It will allow the potential identification of new classes of chemicals, metabolites and degradation products, and the categorization of products based on several indices (e.g., molecular formula, hydrophilicity/polarity, type of degradation mechanism). Developing specific indices to characterize families of compounds will help getting faster results from HRMS measurements, without reducing the level of details obtained by HRMS analysis. The originality of the WaterOmics project lies in this global approach, by avoiding the time-consuming and complex steps of molecular structure identification.

This approach will be tested through the evaluation of performances of advanced wastewater treatment processes towards the elimination of organic micropollutants. Micropollutant transformation during various oxidation processes (ozonation, UV photolysis, chlorination, peracids) will be studied at the lab-scale and pilot-scale. The performance of major wastewater treatment plants of the Paris conurbation (including the largest European plant) will also be evaluated for the removal of micropollutants. Results will provide remediation strategies to limit the impact of micropollutants on the environment. An important sampling program in wastewater plants, discharges and urban rivers will be performed in collaboration with a major wastewater plant operator (SIAAP). The long-term impacts of urban water discharges in the environment will be followed, and multiple HRMS fingerprints from various urban waters (i.e., wastewater, runoff and fresh waters) will be acquired in a database. The validation of methods and indices and their robustness by investigating a broad range of water matrices will be essential. Relationships between classes of urban contaminants and the toxicity of samples will be established to get a comprehensive view of the exposome from urban water samples.

This project will provide innovative tools to characterize and monitor the behavior of organic compounds in water systems and their toxic impacts, which will benefit to the water community, from researchers to operational institutions and industrial structures involved in water management and treatment.

This project is associated to the Observatory of URban Pollutants (OPUR).

Publications and communications

  • HUYNH N., MEBOLD E., MOILLERON R., ROUX J. L. “Comparison of three chromatographic columns using the advantage of ion mobility for non-target analysis of organic micropollutants in wastewater samples.”. In : [En ligne]. SETAC North America 41st Annual Meeting. [s.l.] : [s.n.], 2020. Disponible sur : < > (consulté le 19 November 2020)

  • HUYNH N., LE ROUX J., MOILLERON R. “Fractionnement d'une eau résiduaire urbaine pour la caractérisation de micropolluants organiques par spectrométrie de masse haute résolution.”. In : GRUTTEE2020 : 13ème congrès international du GRUTTEE [En ligne]. GRUTTEE2020 : 13ème congrès international du GRUTTEE. Rennes, France : [s.n.], 2020. Disponible sur : < > (consulté le 2 March 2020)

  • HUYNH N., FIDJI S., GARRIGUE-ANTAR L., MORIN C., CAUPOS E., GROMAIRE M. - C., MOILLERON R., ROUX J. L. “Evaluation of road runoff treatment systems performance by non-target screening combined with ecotoxicological studies.”. In : [En ligne]. 16th Annual Workshop On Emerging High-Resolution Mass Spectrometry And LC-MS/MS Applications In Environmental Analyses And Food Safety. [s.l.] : [s.n.], 2020. Disponible sur : < > (consulté le 19 November 2020)

  • NIHEMAITI M., HUYNH N., MAILLER R., MÈCHE P., ROCHER V., LE ROUX J. “Screening of micropollutants and their transformation products in performic acid treated wastewater.”. In : 11th Micropol & Ecohazard Conference 2019 [En ligne]. Seoul, South Korea : [s.n.], 2019. Disponible sur : < > (consulté le no date)

  • NIHEMAITI M., HUYNH N., MAILLER R., MÈCHE P., ROCHER V., LE ROUX J. “Screening of micropollutants and their transformation products in performic acid treated wastewater.”. In : Wasserchemischen Gesellschaft 8th Late Summer Workshop ”Chemical and biological transformation processes and tools for their investigation” [En ligne]. Haltern am See, Germany : [s.n.], 2019. Disponible sur : < > (consulté le no date)
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